Green Flourescent Protein or GFP's are responsible for a sea jelly's ability to glow in the deepest parts of the ocean. In this experiment, we will see how plasmids move genes from one organism to another. This process allows bacteria in nature to adapt to their environments by transferring plasmids back and fourth with the beneficial genes and makea them better suited for the new conditions. This phenomena is what allows bacteria to gain a fast resistance to antibiotics, thus making them virtually unstoppable.
The pGLO that we will be using in our experiment encodes the gene for the Green Fluorescent Protein (GFP) and also incorporates a gene regulation system which controls the fluorescent protein. In this lab, we will perform the genetic transformation using plasmids.
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ReplyDeleteGreat background info, but you need to talk about the lab itself to bring this up to an A. Discuss the procedure, variables, controls, and predictions.